Coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings.
Identifieur interne : 000266 ( Main/Exploration ); précédent : 000265; suivant : 000267Coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings.
Auteurs : Mitchell M. Mccartney [États-Unis] ; Sierra L. Spitulski [États-Unis] ; Alberto Pasamontes [États-Unis] ; Daniel J. Peirano [États-Unis] ; Michael J. Schirle [États-Unis] ; Raquel Cumeras [États-Unis] ; Jason D. Simmons [États-Unis] ; Jeffrey L. Ware [États-Unis] ; Joshua F. Brown [États-Unis] ; Alexandria J Y. Poh [États-Unis] ; Seth C. Dike [États-Unis] ; Elizabeth K. Foster [États-Unis] ; Kristine E. Godfrey [États-Unis] ; Cristina E. Davis [États-Unis]Source :
- Talanta [ 1873-3573 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Volatile Organic Compounds.
- chemistry : Citrus, Volatile Organic Compounds.
- growth & development : Citrus.
- chemical , isolation & purification : Volatile Organic Compounds.
- methods : Spectrum Analysis.
- Environment, Controlled, Humidity.
Abstract
Volatile organic compounds (VOCs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. In agricultural systems, these VOCs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. Previously we reported a portable gas chromatography/differential mobility spectrometry (GC/DMS) system that could distinguish VOC profiles of pathogen-infected citrus from healthy trees before visual symptoms of disease were present. These measurements were taken directly from canopies in the field, but the sampling and analysis protocol did not readily transfer to a controlled greenhouse study where the ambient background air was saturated with volatiles contained in the facility. In this study, we describe for the first time a branch enclosure uniquely coupled with GC/DMS to isolate and measure plant volatiles. To test our system, we sought to replicate our field experiment within a contained greenhouse and distinguish the VOC profiles of healthy versus citrus infected with Candidatus Liberibacter asiaticus. We indeed confirm the ability to track infection-related trace biogenic VOCs using our sampling system and method and we now show this difference in Lisbon lemons (Citrus×limon L. Burm. f.), a varietal not previously reported. Furthermore, the system differentiates the volatile profiles of Lisbon lemons from Washington navels [Citrus sinensis (L.) Osbeck] and also from Tango mandarins (Citrus reticulata Blanco). Based on this evidence, we believe this enclosure-GC/DMS system is adaptable to other volatile-based investigations of plant diseases in greenhouses or other contained settings, and this system may be helpful for basic science research studies of infection mechanisms.
DOI: 10.1016/j.talanta.2015.08.039
PubMed: 26695246
Affiliations:
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Le document en format XML
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Electronic address: cedavis@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26695246</idno><idno type="pmid">26695246</idno><idno type="doi">10.1016/j.talanta.2015.08.039</idno><idno type="wicri:Area/PubMed/Corpus">000097</idno><idno type="wicri:Area/PubMed/Curation">000097</idno><idno type="wicri:Area/PubMed/Checkpoint">000097</idno><idno type="wicri:Area/Ncbi/Merge">001E27</idno><idno type="wicri:Area/Ncbi/Curation">001E27</idno><idno type="wicri:Area/Ncbi/Checkpoint">001E27</idno><idno type="wicri:Area/Main/Merge">000266</idno><idno type="wicri:Area/Main/Curation">000266</idno><idno type="wicri:Area/Main/Exploration">000266</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings.</title><author><name sortKey="Mccartney, Mitchell M" sort="Mccartney, Mitchell M" uniqKey="Mccartney M" first="Mitchell M" last="Mccartney">Mitchell M. Mccartney</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Spitulski, Sierra L" sort="Spitulski, Sierra L" uniqKey="Spitulski S" first="Sierra L" last="Spitulski">Sierra L. Spitulski</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Pasamontes, Alberto" sort="Pasamontes, Alberto" uniqKey="Pasamontes A" first="Alberto" last="Pasamontes">Alberto Pasamontes</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Peirano, Daniel J" sort="Peirano, Daniel J" uniqKey="Peirano D" first="Daniel J" last="Peirano">Daniel J. Peirano</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Schirle, Michael J" sort="Schirle, Michael J" uniqKey="Schirle M" first="Michael J" last="Schirle">Michael J. Schirle</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Cumeras, Raquel" sort="Cumeras, Raquel" uniqKey="Cumeras R" first="Raquel" last="Cumeras">Raquel Cumeras</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Simmons, Jason D" sort="Simmons, Jason D" uniqKey="Simmons J" first="Jason D" last="Simmons">Jason D. Simmons</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Ware, Jeffrey L" sort="Ware, Jeffrey L" uniqKey="Ware J" first="Jeffrey L" last="Ware">Jeffrey L. Ware</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Brown, Joshua F" sort="Brown, Joshua F" uniqKey="Brown J" first="Joshua F" last="Brown">Joshua F. Brown</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Poh, Alexandria J Y" sort="Poh, Alexandria J Y" uniqKey="Poh A" first="Alexandria J Y" last="Poh">Alexandria J Y. Poh</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Dike, Seth C" sort="Dike, Seth C" uniqKey="Dike S" first="Seth C" last="Dike">Seth C. Dike</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Foster, Elizabeth K" sort="Foster, Elizabeth K" uniqKey="Foster E" first="Elizabeth K" last="Foster">Elizabeth K. Foster</name><affiliation wicri:level="2"><nlm:affiliation>Contained Research Facility, University of California, Davis, 555 Hopkins Road, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Contained Research Facility, University of California, Davis, 555 Hopkins Road, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Godfrey, Kristine E" sort="Godfrey, Kristine E" uniqKey="Godfrey K" first="Kristine E" last="Godfrey">Kristine E. Godfrey</name><affiliation wicri:level="2"><nlm:affiliation>Contained Research Facility, University of California, Davis, 555 Hopkins Road, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Contained Research Facility, University of California, Davis, 555 Hopkins Road, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Davis, Cristina E" sort="Davis, Cristina E" uniqKey="Davis C" first="Cristina E" last="Davis">Cristina E. Davis</name><affiliation wicri:level="2"><nlm:affiliation>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Electronic address: cedavis@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Talanta</title><idno type="eISSN">1873-3573</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (chemistry)</term><term>Citrus (growth & development)</term><term>Environment, Controlled</term><term>Humidity</term><term>Spectrum Analysis (methods)</term><term>Volatile Organic Compounds (analysis)</term><term>Volatile Organic Compounds (chemistry)</term><term>Volatile Organic Compounds (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Volatile Organic Compounds</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term><term>Volatile Organic Compounds</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Volatile Organic Compounds</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrum Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Environment, Controlled</term><term>Humidity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Volatile organic compounds (VOCs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. In agricultural systems, these VOCs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. Previously we reported a portable gas chromatography/differential mobility spectrometry (GC/DMS) system that could distinguish VOC profiles of pathogen-infected citrus from healthy trees before visual symptoms of disease were present. These measurements were taken directly from canopies in the field, but the sampling and analysis protocol did not readily transfer to a controlled greenhouse study where the ambient background air was saturated with volatiles contained in the facility. In this study, we describe for the first time a branch enclosure uniquely coupled with GC/DMS to isolate and measure plant volatiles. To test our system, we sought to replicate our field experiment within a contained greenhouse and distinguish the VOC profiles of healthy versus citrus infected with Candidatus Liberibacter asiaticus. We indeed confirm the ability to track infection-related trace biogenic VOCs using our sampling system and method and we now show this difference in Lisbon lemons (Citrus×limon L. Burm. f.), a varietal not previously reported. Furthermore, the system differentiates the volatile profiles of Lisbon lemons from Washington navels [Citrus sinensis (L.) Osbeck] and also from Tango mandarins (Citrus reticulata Blanco). Based on this evidence, we believe this enclosure-GC/DMS system is adaptable to other volatile-based investigations of plant diseases in greenhouses or other contained settings, and this system may be helpful for basic science research studies of infection mechanisms.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Mccartney, Mitchell M" sort="Mccartney, Mitchell M" uniqKey="Mccartney M" first="Mitchell M" last="Mccartney">Mitchell M. Mccartney</name></region><name sortKey="Brown, Joshua F" sort="Brown, Joshua F" uniqKey="Brown J" first="Joshua F" last="Brown">Joshua F. Brown</name><name sortKey="Cumeras, Raquel" sort="Cumeras, Raquel" uniqKey="Cumeras R" first="Raquel" last="Cumeras">Raquel Cumeras</name><name sortKey="Davis, Cristina E" sort="Davis, Cristina E" uniqKey="Davis C" first="Cristina E" last="Davis">Cristina E. Davis</name><name sortKey="Dike, Seth C" sort="Dike, Seth C" uniqKey="Dike S" first="Seth C" last="Dike">Seth C. Dike</name><name sortKey="Foster, Elizabeth K" sort="Foster, Elizabeth K" uniqKey="Foster E" first="Elizabeth K" last="Foster">Elizabeth K. Foster</name><name sortKey="Godfrey, Kristine E" sort="Godfrey, Kristine E" uniqKey="Godfrey K" first="Kristine E" last="Godfrey">Kristine E. Godfrey</name><name sortKey="Pasamontes, Alberto" sort="Pasamontes, Alberto" uniqKey="Pasamontes A" first="Alberto" last="Pasamontes">Alberto Pasamontes</name><name sortKey="Peirano, Daniel J" sort="Peirano, Daniel J" uniqKey="Peirano D" first="Daniel J" last="Peirano">Daniel J. Peirano</name><name sortKey="Poh, Alexandria J Y" sort="Poh, Alexandria J Y" uniqKey="Poh A" first="Alexandria J Y" last="Poh">Alexandria J Y. Poh</name><name sortKey="Schirle, Michael J" sort="Schirle, Michael J" uniqKey="Schirle M" first="Michael J" last="Schirle">Michael J. Schirle</name><name sortKey="Simmons, Jason D" sort="Simmons, Jason D" uniqKey="Simmons J" first="Jason D" last="Simmons">Jason D. Simmons</name><name sortKey="Spitulski, Sierra L" sort="Spitulski, Sierra L" uniqKey="Spitulski S" first="Sierra L" last="Spitulski">Sierra L. Spitulski</name><name sortKey="Ware, Jeffrey L" sort="Ware, Jeffrey L" uniqKey="Ware J" first="Jeffrey L" last="Ware">Jeffrey L. Ware</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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